human epithelial melanoma cell line a2058  (ATCC)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058).

doi: 10.1016/j.biopha.2021.112438

Figure Lengend Snippet: Fig. 2. – Mitochondrial activity (%) of A2058 cells after 24 and 72 h of treatment with bullfrog oil (BFO), 5 - fluorouracil (5-FU), bullfrog oil nanoemulsion, (BFONe), bullfrog oil microemulsion (BFOMe), and bullfrog oil nanocapsules (BFONc). * Sample statistically different from the control (p < 0.05).

Article Snippet: The Human epithelial melanoma cell line A2058 (ATCC® CRL-11147TM) and the Bullfrog (Rana catesbeiana Shaw) adipose tissue were kindly donated by Prof. Dr. José Alexandre Barbuto from the Institute of Biomedical Sciences, Tumor Immunology Laboratory – São Paulo University (USP) (São Paulo, SP, Brazil) and Asmara Produtos Naturais Ltda. (Natal, RN, Brazil), respectively.

Techniques: Activity Assay, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058).

doi: 10.1016/j.biopha.2021.112438

Figure Lengend Snippet: Fig. 3. – A2058 cell death evaluation after 72 h of bullfrog oil treatment at 100 µg.mL−1. A: Untreated cells used as control; B: Cells treated with bullfrog oil; C: Cells treated with 5 – fluorouracil; D: Cells treated with bullfrog oil nanoemulsion (BFONe); E: Cells treated with bullfrog oil microemulsion (BFOMe); F: Cells treated with bullfrog oil nanocapsules (BFONc). G: Bar graph representation of average cell percentage in each cell death stage after treatment. Q1: necrosis (Annexin-V-/PI+); Q2: late apoptosis (Annexin-V+/PI+); Q3: early apoptosis (Annexin-V+/PI-); Q4: viable cells (Annexin-V-/PI-). PI: Propidium Iodide; FITC-A: Annexin V-FITC fluorescence intensity; PerCP-Cy5–5-A: Iodide propidium fluorescence intensity. n = 6. *Significant difference compared to the control (p < 0.05).

Article Snippet: The Human epithelial melanoma cell line A2058 (ATCC® CRL-11147TM) and the Bullfrog (Rana catesbeiana Shaw) adipose tissue were kindly donated by Prof. Dr. José Alexandre Barbuto from the Institute of Biomedical Sciences, Tumor Immunology Laboratory – São Paulo University (USP) (São Paulo, SP, Brazil) and Asmara Produtos Naturais Ltda. (Natal, RN, Brazil), respectively.

Techniques: Control, Fluorescence

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058).

doi: 10.1016/j.biopha.2021.112438

Figure Lengend Snippet: Fig. 4. – Intracellular ROS levels of A2058 cells after 72 h of bullfrog oil treatment at 100 µg.mL−1. BFO (bullfrog oil), BFONe (bullfrog oil nano emulsion), BFOMe (bullfrog oil microemulsion), BFONc (bullfrog oil nano capsules). * Sample statistically different from the control (p < 0.05).

Article Snippet: The Human epithelial melanoma cell line A2058 (ATCC® CRL-11147TM) and the Bullfrog (Rana catesbeiana Shaw) adipose tissue were kindly donated by Prof. Dr. José Alexandre Barbuto from the Institute of Biomedical Sciences, Tumor Immunology Laboratory – São Paulo University (USP) (São Paulo, SP, Brazil) and Asmara Produtos Naturais Ltda. (Natal, RN, Brazil), respectively.

Techniques: Nano-Emulsion, Capsules, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Nanostructured systems increase the in vitro cytotoxic effect of bullfrog oil in human melanoma cells (A2058).

doi: 10.1016/j.biopha.2021.112438

Figure Lengend Snippet: Fig. 5. - Representative image of fluorescent microscopy of A2058 cells treated with different samples over 24 h: left panel 6 h of treatment; middle panel 12 h of treatment; right panel 24 h of treatment. Cell structures stained with Hoechst (Hoe) aqueous solution (blue fluorescence) and bullfrog oil Nile red (NR) stained (red fluorescence). Contrast images (Cont) with purple fluorescence (merge of Hoe and NR). Control (untreated cells); BFO (bullfrog oil), BFONe (bullfrog oil nano emulsion), BFOMe (bullfrog oil microemulsion), BFONc (bullfrog oil nanocapsules). Scale bars represent 20 µm.

Article Snippet: The Human epithelial melanoma cell line A2058 (ATCC® CRL-11147TM) and the Bullfrog (Rana catesbeiana Shaw) adipose tissue were kindly donated by Prof. Dr. José Alexandre Barbuto from the Institute of Biomedical Sciences, Tumor Immunology Laboratory – São Paulo University (USP) (São Paulo, SP, Brazil) and Asmara Produtos Naturais Ltda. (Natal, RN, Brazil), respectively.

Techniques: Microscopy, Staining, Fluorescence, Control, Nano-Emulsion

Journal: Nutrients

Article Title: Biological Effect of Food for Special Medical Purposes (Nutramil TM Complex) on Melanoma Cells in In Vitro Study

doi: 10.3390/nu16244287

Figure Lengend Snippet: The effect of Nutramil TM Complex and Nutramil TM Complex without calcium caseinate on mRNA expression of genes in human melanoma WM-115 and WM-266-4 cells lines.

Article Snippet: This research was conducted with use of human primary melanoma cell line WM115 (ATCC ® CRL-1675TM), human malignant melanoma cell line WM266-4 (ATCC ® CRL-1676TM), and human foreskin fibroblast cell line BJ (ATCC ® CRL-2522TM).

Techniques: Expressing

Journal: Cell Genomics

Article Title: A cis -regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation

doi: 10.1016/j.xgen.2022.100191

Figure Lengend Snippet:

Article Snippet: WM-266-4 human malignant melanoma cell line was obtained from ATCC (CRL-1676) and grown in DMEM F:12 (ThermoFisher, 11995-065) supplemented with 10% FBS.

Techniques: Virus, Recombinant, Reverse Transcription, SYBR Green Assay, Staining, Library Quantification, HiChIP, Software